SureSawit™ KARMA Test
CLONE WITH CONFIDENCE
- Verify somaclonal varients
- Improve oil palm productivity
- Decrease land pressure
The recent discovery of the epigenetic cause of mantling makes it possible to develop a DNA test for the low-yielding mantled phenotype. The SureSawit KARMA test enables the validation of somaclonal variants at the nursery stage so that mantled trees can be culled and only high performing palms are cultivated. The planting of high performing clonal palms has the potential to produce 20 to 30 percent more oil on the same planted area as palms grown from seedlings.
The test starts with the SureSawit Sample Collection Kit. The SureSawit Sample Collection Kit includes everything you need for easy, trackable collection of oil palm leaves for downstream processing. The patented leaf punch and tree tag facilitate sample collection and tree labeling from a nursery palm, juvenile palm or adult palm. The punch and tag include barcodes for accurate tracking of leaf sample to tree.
DNA is extracted from the leaf samples using a proprietary DNA isolation protocol. The DNA is then genotyped on the Douglas Scientific IntelliQube, based on the Array Tape® platform. This cutting edge, automated system includes liquid handling, thermal cycling, detection and analysis. The addition of the Intellicycler® PCR Water Bath Thermocycler provides even more genotyping capacity, allowing Orion Biosains to process tens of thousands of samples per day!